ABSTRACT
OBJECTIVE:To extract the feature of Achyranthes bidentata and Cyathula officinalis,and to establish image recog-nition method. METHODS:The microscopic image stitching of A. bidentata and C. officinalis was implemented by MATLAB. The color,invariant moment,stripes and the features of vascular bundle in cross section were extracted. The data was organized into da-ta matrix,and then data matrix was standardized by Zscore function;principal components were analyzed through Princomp func-tion. BP nerve network recognition mode was adopted. RESULTS:The microstructures in the micro images of the samples were kept integrated. The measured data of 27 characteristics were acquired in each group of sample. Through principal component analy-sis,the parameters of 11 main components were selected to establish BP never network. The average recognition rate of BP nerve network was 100% between 2 medicinal material relatives (n=50). CONCLUSIONS:The method can be used for micro-image auto Stitching of Chinese medicinal materials and image recognition of A. bidentata and C. officinalis.
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[Objectivs] To investigate the principle of AgNO3-processed silica gel chromatography in isolation and purification of mixtures with carbon-carbon double bonds (CCDB) from herbal medicine. [Methods] AgN03-processed gel chromatography was used for the isolation of mixtures with carbon-carbon double bonds in the volatile oils and active components from Pteris semipinnata L. ; silver-ion complexion chromatography was applied to isolate arachidom'c acid from lipids. The principle of AgNO3-processed silica gel chromatography was analyzed by coordination theory. [Results] Stable AgNO3 coordinate complexes were obtained. [ Conclusion ] The principle of the formation of stable AgNO3 coordinate complexes may be: (1) the stability will increase with CCDB in free space; (2) exocyclic CCDB is more stable than that of intra-cyclic CCDB; (3) cis-form CCDB is more stable than that of trans-form CCDB; (4) terminal CCDB is more stable than that of cis-form CCDB; (5) aromatic hydrocarbon with multiple benzene rings is more stable than that with single ber zene ring. The possible principle will benefit to the isolation and purification of the mixtures with CCDB, which have similar structure and physiochemical property and are difficult to be isolated and purified by common chromatography and extriction method.
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The identification of Radix Morindae Officinalis, Radix Rehmanniae Preparata, Herba Epimedii, Radix Ginseng, Radix Achyranthis Bidentatae, Rhizoma Dioscoreae in Baji Bushen decoction was carried out respectively by TLC. The content of icariin in the preparation was determined by HPLC. Chromatocolumn was adopted, methanol - 0. 5 % HClO4 (55: 45) as the mobile phase, flow - rate at 0.8 mL/min, column temperature at 40℃, detection wavelength at 270nm, the mean recovery is 97. 36%and RSD is 2.61% (N=5). It is indicated that the methods can be used for the quality control of Baji Bushen decoction.
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Objective To establish the standard for the quality control of Compound Huangqin Tablets. Methods The identification of Radix Scutellariae,Rhizoma Polygoni Cuspidati and Caulis Mahoniae in compound Huangqin tablets were carried out by TLC.The content of baicalin in the preparation was determined by HPLC.Chromatocolumn was adopted,methanol-0.4 %H3PO4(50 ∶50) used as the mobile phase,flowrate 1.4mL/min,column temperature at 40 ℃and detection wavelength 278 nm . Results The TLC spots were highly clear without the interference of negative control and were reproductive. The calibration curve for baicalin was linear (r=0.9996) in the range of 0.12～0.72 ?g and the mean recovery was 97.17 %and RSD=1.78 %(n=5). Conclusion This method can be used for the quality control of Compound Huangqin Tablets.
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Objective: To establish a HPLC method for the determination of synephrine in Dachengqi Granules (Radix et Rhizoma Rhei, Fructus Aurantii Immaturus, Cortex Magnoliae Officinalis, Natrii Sulfas). Methods: A E. Merck LiChrospher 100RP 18 column was /used. The mobile phase was methanol water (contained 0.18% H 3PO 4 and 0.22% C 12 H 25 SO 4Na) (55∶45). The detection wavelength was at 274nm.Results: The linear range of synephrine was 0.14?g～1.54?g, r =0.9998. The average recovery was 98.1%, RSD =2.1% ( n =5).Conclusion: The result is accurate and the reproducibility is good. This method can be used for the determination of synephrine in Dachengqi Granules.